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. 2006 Sep 4;3:57. doi: 10.1186/1742-4690-3-57

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Copyright © 2006 Westerhout et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sequence-specific inhibition in shNef-expressing cells. a) Schematic of RNAi-mediated targeting of mRNA with the shNef target sequence (gray box) in shNef-expressing SupT1 cells. b) SupT1 cells stably expressing shNef (+ shNef) or control SupT1 cells (- shNef) were transfected with luciferase reporter constructs that contain the complete shNef target sequence (pGL3-Nef) or not (pGL3-R2). The mean values obtained in two independent experiments are shown. Values measured in the control transfection (- shNef) were set at 100% for each reporter construct.