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. 2006 Sep 4;3:57. doi: 10.1186/1742-4690-3-57

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Copyright © 2006 Westerhout et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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No inhibition of lentiviral transduction with virions containing the HIV-1 Envelope. SupT1 cells stably expressing shNef (+ shNef) or control SupT1 cells (- shNef) were transduced at an m.o.i. of 0.03, 0.2 or 0.5 with either the control (JS1) or the shNef target sequence containing (wt-Nef) lentiviral vector with an HIV-1 envelope protein. Infected cells were analyzed by GFP-FACS. The control values (- shNef) were set at 100% for each infection. The mean values of two independent experiments are shown.