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. Author manuscript; available in PMC: 2006 Sep 18.
Published in final edited form as: J Thromb Haemost. 2005 Dec;3(12):2773–2783. doi: 10.1111/j.1538-7836.2005.01593.x

Figure 1. Immunoprecipitation and immunoblotting of the transfected HEK 293 cells.

Figure 1

Cell lines were solubilized in lysis buffer and immunoprecipitated with an antibody to the V5 epitope tag of the αIIb subunit. Immunocomplexes were analyzed by immunoblotting under reducing (αIIb) or non-reducing (β3) conditions using an antibody to αIIb (CA3, top) or β3 (CD61, bottom). Pro-αIIb, mature αIIb and β3 are indicated by arrows.