. Author manuscript; available in PMC: 2006 Sep 18.

Published in final edited form as: Nature. 2005 Dec 4;439(7074):344–348. doi: 10.1038/nature04388

Table 1.

Genomic diversity for wildtype, G64S and G64S^eQS populations

Virus	Total number of mutations^a	Mutation per genome	gua^r variants^b
wildtype	13/50,700	1.91	62 ± 6
G64S	2/50,700	0.31	22 ± 8
G64S^eQS	14/50,700	2.06	56 ± 6
^c
wildtype^b	12/48,588	1.84	ND
G64S^b	6/69,000	0.65	ND
G64S^eQS-b	6/50,700	0.88	ND

Number of mutations observed over the total number of nucleotides sequenced. To determine the mutation frequency in each poliovirus population, 24 independent poliovirus cDNA clones were obtained. Poliovirus cDNAs were generated by RT-PCR from plaque isolated viral RNA. A significant difference was observed between WT and G64S (p<0.002) based on a Mann Whitney U test. In contrast, no significant difference was observed between WT and G64S^eQS (p<0.222).

Per 10⁶ plaque-forming units (PFU), Mean ± s.d. of 6 experiments. Significance testing yielded p<0.001 by ANOVA.

wildtype^b, G64S^b, G64S^eQS-B re-isolated from infected brain. Number of Gua^r variants was not determined.