Figure 4.

Rapid genotype analysis of bpk. (A) Schematic representation of Bicc1 exon 22 showing the location of the bpk mutation (CG insertion) and creation of a unique BstU1 restriction site. (B) PCR amplification with primers flanking the mutation site, followed by BstU1 restriction digest of PCR products clearly distinguishes between wild-type, homozygous, and heterozygous DNA samples. The wild-type allele yields a 213 bp product, which is not cut by BstU1. The bpk mutant allele yields a 215 bp product, which is cut into 125 bp and 90 bp fragments. WT, wild type; CYS, cystic; HET, heterozygote.