Table 2.

Running methods and temperature used for DHPLC mutation detection

Gene-Exon (bp)	Starting Gradient (%B)	Temperature (°C)
p53-4	58.2	60.9/62.5
p53-5I	50	64.4
p53-5II	52.9	65.1
p53-6	53.1	61.5
p53-7	53.5	61.7/63.4/64.3
p53-8	54.6	59.8/62.8
p53-9	50.5	58.6/59.6
c-kit-9	56.5	55.3/58.5/59.5
c-kit-11	51.2	57.7/58.7
c-kit-13	55.4	56.7/58.7/59.3
c-kit-17	54.2	56.5/58
EGFR-18 (400)	57	60.9/62.9
EGFR-19 (372)	56.5	60.1
EGFR-20 (409)	57.2	61.2/63.4
EGFR-21 (415)	57.3	60.8