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. 2006 Sep 13;399(Pt 1):141–150. doi: 10.1042/BJ20060194

Figure 10. Association of endogenous Fes with KAP-1 in HL-60 cells.

Figure 10

(A) HL-60 myeloid leukaemia cells were treated with retinoic acid (RA) or DMSO to induce terminal differentiation or left untreated (Control). The extent of differentiation was assessed 4 days later as the percentage of cells able to reduce NBT. Results are mean±S.D., n=6. (B) Cell lysates from control and differentiated cells in (A) were probed for Fes and KAP-1 protein levels by immunoblotting as described in the Materials and Methods section. An actin blot was also performed as a loading control. (C) Fes was immunoprecipitated from HL-60 cell lysates and probed for associated KAP-1 as well as Fes by immunoblotting. This experiment was repeated five times, and the level of Fes–KAP-1 interaction shown was observed in two of these experiments. KAP-1 recovery was weaker or absent in the other trials, which is likely to reflect heterogeneity in the level of Fes protein available for immunoprecipitation.