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. 2006 Sep 13;399(Pt 1):141–150. doi: 10.1042/BJ20060194

Figure 2. KAP-1 selectively interacts with the Fes CC2 domain.

Figure 2

Yeast strain AH109 was co-transformed with the indicated pairs of plasmids and grown either on plates lacking leucine and tryptophan (−L−W) or on plates lacking leucine, tryptophan, adenine and histidine (−L−W−A−H; upper panel). Growth on −L−W plates selects for the presence of both plasmids and growth on −L−W−A−H plates is indicative of protein–protein interaction. Results from four independent transformants are shown for each combination. Yeast colonies from −L−W plates in the upper panel were grown in liquid medium and cell lysates were immunoblotted with Myc (middle panel) or HA antibodies (lower panel) to detect the DBD and AD fusions respectively. The positions of the fusion proteins are indicated on the left. Data are representative of three independent experiments.