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. 2006 Sep 13;399(Pt 1):141–150. doi: 10.1042/BJ20060194

Figure 3. KAP-1 interacts with full-length Fes.

Figure 3

Yeast strain AH109 was co-transformed with the indicated pairs of plasmids. Colonies were replated on −L−W plates to verify transformation of both plasmids and on −L−W−A−H plates to test for protein–protein interaction (upper panel). Results from four independent transformants are shown for each combination. Colonies from −L−W plates were grown in liquid medium and clarified lysates were subjected to immunoblot analyses utilizing Myc antibodies to detect DBD-fusion proteins and HA antibodies to visualize AD-fusion proteins as indicated (lower panels). The positions of the AD and DBD proteins are indicated on the left. Data are representative of three independent experiments.