Table 3.
A subset of tryptic peptides from glyceraldehyde-3-phosphate dehydrogenase C subunit (At3g04120) that were identified by mass spectrometry and subsequent database search (14N-search, see methods for details). For each peptide the expected mass difference was calculated based on the measured mass to charge ratio (MCR). Asterisks mark those peptides that have also been identified in the database search with 15N as fixed modification (15N-search, see Methods for details)
| Sequence | measured mass 14N | MCR 14N [Th] | Charge | calculated mass difference | calculated mass 15N | MCR 15N [Th] |
| AASFNIIPSSTGAAK | 1434.42 | 718.22 | 2 | 16.95 | 1451.37 | 726.69 |
| DAPMFVVGVNEHEYK | 1733.42 | 867.72 | 2 | 18.94 | 1752.36 | 877.18 |
| FGIVEGLMTTVHSITATQK * | 2033.69 | 1017.85 | 2 | 18.94 | 2052.63 | 1027.32 |
| GILGYTEDDVVSTDFVGDNR | 2170.55 | 1086.28 | 2 | 23.93 | 2194.48 | 1098.24 |
| LVSWYDNEWGYSSR | 1760.47 | 881.24 | 2 | 19.94 | 1780.41 | 891.20 |
| SDLDIVSNASCTTNCLAPLAK | 2248.71 | 1125.36 | 2 | 23.93 | 2272.64 | 1137.32 |
| VPTVDVSVVDLTVR * | 1497.55 | 749.78 | 2 | 16.95 | 1514.50 | 758.25 |
| YDSVHGQWK | 1118.39 | 560.20 | 2 | 13.96 | 1132.35 | 567.17 |