Table 5.
Overview of the costs of SILAC labeling and full 15N-labeling for plant cell culturesa. Depending on the compounds and concentrations used in the medium, K15NO3 labeling is 7 to 12 times cheaper per liter medium.
| Compound | Amount in package (g) | Cost per package (€) | Cost per g (€) | Final concentration in medium (mM) | Price per L (€) | Cost Factor |
| 13C6-R | 0.100 | 750 b | 7500 | 0.8 c | 972 | 13 |
| 13C6-K | 0.100 | 800 b | 8000 | 0.8 c | 1037 | 13 |
| D3-L | 0.100 | 550 b | 5500 | 0.8 c | 590 | 8 |
| K15NO3 | 1 | 75 b | 75 | 10 d | 77 | 1 |
a for comparison, the iTRAQ Multiplex Kit costs approximately 1000 € (Applied Biosystems, Germany), allowing for 10 pairwise labelling reactions.
b approximate prices according to SigmaAldrich, Germany
c most efficient concentration used in Gruhler et al. 2005, MCP [35]
d as used in this study