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. 2006 Aug 31;25(18):4263–4270. doi: 10.1038/sj.emboj.7601304

Figure 3.

Figure 3

Dazl travels via the microtubule network. (A) The COS7 cells transfected with pHA-Dazl were treated with either 10 μM nocodazole (Noc) or 1 μM cytochalasin D (CtD) for 1 h and immunostained with antibodies against Dazl, α-tubulin and Dlc. Actin was stained with phaloidin. Scale bars, 10 μm. (B) Subcellular distribution of HA-Dazl in the COS7 cells was observed in the absence or presence of GFP-Dynamitin. Scale bar, 10 μm. (C) Association of Dazl with microtubules. The soluble fraction of the mouse testicular lysates (Lys) was treated with 20 μM taxol and 0.5 mM GTP and centrifuged to separate the precipitate (Ppt) from the supernatant (Spt). Immunoblotting analyses were performed using antibodies against α-tubulin, Dic, Dazl and the Rbm proteins.