Figure 1.
RIG-I induces type I IFN in EBV-infected cells. EBV-positive Mutu cells (Mutu(+)) and EBV-negative Mutu cells (Mutu(−)) (5 × 106 each) were transfected with 30 μg of GFP-tagged RIG-I plasmid or control GFP plasmid, and expression of IFNs and ISG56 was examined at the designated time by RT–PCR. (A) Immunoblot analysis for detection of RIG-I. The blots were probed with anti-RIG-I antibody (left panel) and anti-GFP antibody (right panel). Cell lysates were prepared after 24 h of transfection and 20 μg of protein sample was loaded per slot. (B) RT–PCR analysis of expression of IFN-α, IFN-β, and ISG56 in Mutu (+) cells. (C) RT–PCR analysis of expression of IFN-α, IFN-β, and ISG56 in Mutu (−) cells. (D) RT–PCR analysis of EBER1 expression in Mutu(+) and Mutu(−) cells.