Figure 3.
EBERs induce type I IFN in RIG-I-expressing cell clones. EBV-negative Daudi cells were transfected with the plasmid carrying GFP-tagged RIG-I or control plasmid carrying the GFP gene only, and cell clones stably expressing RIG-I/GFP or GFP were isolated. They (5 × 106 each) were transfected with 30 μg of EBER plasmid, and expression of type I IFN was examined at the designated time by RT–PCR. (A) Immunoblot analysis for detection of RIG-I. The blots were probed with the anti-GFP antibody. Protein samples amounting to 20 μg was loaded per slot. (B) RT–PCR analysis for detection of IFN-β.