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. 2006 Aug 31;25(18):4207–4214. doi: 10.1038/sj.emboj.7601314

Figure 4.

Figure 4

Both EBER1 and EBER2 activate RIG-I to induce type I IFN. (A) Analysis of in vitro-synthesized EBER1 and EBER2 in 5% denaturing PAGE before (left panel) and after (middle panel) RNase A treatment. RT–PCR analysis of EBER1 cDNA with EBER1 and EBER2 primers and also EBER2 cDNA with EBER2 and EBER1 primers (right panel). (B) RT–PCR analysis of expression of IFN-β, ISG56, and ISG15 after transfection of EBER1 and EBER2. RIG-I- or GFP- (5 × 106 each) expressing stable clones were transfected with 30 μg of EBER1 or EBER2, separately or in combination (1:1). Expression of IFN-β and ISGs was examined after 6 and 24 h of transfection.