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. 2006 Aug 31;25(18):4207–4214. doi: 10.1038/sj.emboj.7601314

Figure 7.

Figure 7

Binding assay for detection of association of EBERs with RIG-I. The full-length RIG-I plasmid (RIG-I/GFP) or control plasmid (containing the GFP gene only) was transfected into EBV-negative Mutu cells (Mutu(−) cells), EBER-positive EBV-infected Mutu cells ((Mutu(+) cells), and EBER-transfected Mutu cells (Mutu/EBER cells). After 48 h, cells were treated with UV irradiation, digested with RNases, and subjected to immunoprecipitation with the anti-GFP antibody. RNA was isolated from immunoprecipitants, and EBER1 and EBER2 were measured by RT–PCR.