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. 2006 Sep 7;25(18):4372–4380. doi: 10.1038/sj.emboj.7601303

Figure 5.

Figure 5

Pharmacology of endocytosed BDNF secretion. (A) BDNF secretion was evaluated by ELISA in perfusates of hippocampal neurons previously exposed to BDNF for 60 min. Glutamate (Glu) application elicited the secretion of endocytosed BDNF in both calcium-containing and calcium-free medium (EGTA). The effect was partially prevented by BAPTA-AM. BDNF secretion was also triggered by caffeine. AMPA- and t-ACPD-induced BDNF secretion was prevented by CNQX and AIDA, respectively. NMDA was not effective either in the presence or absence of APV. (B) BDNF secretion was induced by NT-4 and NT-3 but not NGF. This effect was prevented by K252a. (C) High-frequency stimulation (50 Hz) triggers BDNF secretion. This effect was prevented by TTX. (D) The NO donor NOR3 administered to BDNF-treated neurons elicited a decrease in BDNF secretion. Conversely, KT5823 triggered BDNF secretion. Data in panels A (n=12), B (n=9), C (n=12) and D (n=6) are means±s.e.m.