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. 2006 Sep 7;25(18):4372–4380. doi: 10.1038/sj.emboj.7601303

Figure 6.

Figure 6

Endocytosed BDNF rescues LTP impaired by protein synthesis inhibition. (A) Field EPSPs evoked in CA1 area by Schaffer collaterals stimulation. TBS induced LTP that is maintained for 180 min (Control) (five slices, five rats). In slices perfused with anisomycin (Aniso) from 30 min before TBS to the end of the recording, LTP persisted for only 70–100 min (five slices, five rats). The effect of anisomycin was fully reversed by applying BDNF from 5 min before to 15 min after TBS (Aniso+BDNF) (six slices, five rats). (B) In slices incubated (pre-inc: Aniso) and perfused (Aniso) with anisomycin throughout the recording, LTP persisted for only 70–100 min (nine slices, seven rats). LTP was rescued in slices preincubated with BDNF (pre-inc: Aniso+BDNF) (12 slices, nine rats), an effect that was prevented by TrkB-Fc (Aniso+TrkB-Fc) (eight slices, six rats). (C) Pretreatment with K252a did not affect LTP maintenance (Control; pre-inc: K252a) (10 slices, seven rats). The presence of anisomycin in both preincubation and perfusion fluid reduced LTP duration to 70–100 min (Aniso; pre-inc: Aniso+K252a) (nine slices, six rats). The blocking action of anisomycin on LTP persisted in slices preincubated with BDNF and K252a (Aniso; pre-inc: Aniso+BDNF+K252a) (11 slices, eight rats). (D) Pretreatment with LY294002 (LY) (five slices, five rats) led to the same effects observed in the presence of K252a (panel C). Data in panels A–D are means±s.e.m. of field EPSP slopes plotted as percentages of the baseline. Representative field EPSP traces recorded before and 180 min after TBS are shown on the top of the panels.