Table 1.
HPLC analysis of tuber carotenoids (ng/g dry weight). Carotenoids were measured via diode array HPLC (see Methods) on a minimum of 4 different tubers from 2 different plants. Fold variation with respect to the wild-type is reported for each carotenoid compound and for each line.
| Line | Phytofluene | Lutein | β-carotene | Zeaxanthin | Antheraxanthin | Violaxanthin | Neoxanthin | Other Xanthophylls | Esters | Total Carotenoids |
| Wild-type | 43.07± 12.78 | 588.01± 108.14 | 3.17± 1.01 | 262.64± 108.08 | 1301.33± 479.34 | 1109.08± 420.06 | 541.02± 98.14 | 89.34± 13.02 | 734.35± 173.04 | 4672.02± 1414.62 |
| Gus 1 | 52.01± 5.87 | 531.46± 153.57 | 3.76± 1.32 | 223.02± 63.04 | 1091.14± 293.24 | 1157.69± 332.49 | 685.32± 204.95 | 81.34± 22.40 | 1270.39± 299.42 | 5096.15± 1376.25 |
| Fold Variation | 1.21 | 0.90 | 1.20 | 0.85 | 0.84 | 1.04 | 1.27 | 0.91 | 1.73 | 1.090 |
| AS-e 1 | 160.59± 8.03 | 725.46± 100.33 | 17.17± 3.59 | 626.28± 187.37 | 2173.42± 97.33 | 2788.91± 170.82 | 303.66± 83.88 | 110.94± 38.43 | 2015.11± 525.48 | 8921.56± 1218.27 |
| Fold Variation | 3.73 | 1.23 | 5.42 | 2.38 | 1.67 | 2.51 | 0.56 | 1.24 | 2.74 | 1.909 |
| AS-e 2 | 248.59± 62.92 | 506.12± 112.22 | 14.35± 3.14 | 669.39± 50.98 | 3045.66± 493.89 | 3320.07± 1061.82 | 1219.54± 20.21 | 158.88± 79.35 | 3089.75± 69.24 | 12272.36± 1961.77 |
| Fold Variation | 5.77 | 0.86 | 4.53 | 2.55 | 2.34 | 2.99 | 2.25 | 1.78 | 4.21 | 2.627 |
| AS-e 3 | 86.97± 6.34 | 866.91± 104.46 | 9.48± 1.84 | 465.48± 146.89 | 1703.35± 150.12 | 2917.70± 720.57 | 945.84± 318.98 | 125.80± 55.78 | 1555.41± 589.078 | 8677.12± 2094.07 |
| Fold Variation | 2.02 | 1.47 | 2.99 | 1.77 | 1.31 | 2.63 | 1.75 | 1.41 | 2.19 | 1.857 |
| AS-e 4 | 193.70± 1.23 | 1044.59± 144.40 | 43.56± 14.24 | 990.83± 197.51 | 2198.54± 559.12 | 2935.03± 797.13 | 612.56± 245.85 | 90.14± 24.62 | 1865.33± 1010.02 | 9974.29± 2994.11 |
| Fold Variation | 4.50 | 1.78 | 13.74 | 3.77 | 1.70 | 2.65 | 1.13 | 1.01 | 2.54 | 2.135 |
| AS-e 5 | 35.44± 5.22 | 510.42± 99.42 | 2.60± 0.92 | 324.83± 111.81 | 1266.71± 78.11 | 1124.44± 409.81 | 421.81± 173.67 | 98.73± 20.30 | 776.25± 87.11 | 4561.23± 986.38 |
| Fold Variation | 0.82 | 0.87 | 0.82 | 1.24 | 0.97 | 1.01 | 0.78 | 1.10 | 1.06 | 0.977 |
| AS-e 6 | 50.21± 8.87 | 469.53± 137.01 | 3.32± 2.01 | 338.81± 87.43 | 1369.53± 263.04 | 1378.81± 359.37 | 746.48± 221.87 | 76.81± 13.37 | 688.91± 118.44 | 5123.42± 1211.42 |
| Fold Variation | 1.17 | 0.80 | 1.05 | 1.29 | 1.05 | 1.24 | 1.38 | 0.86 | 0.99 | 1.097 |