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Electropherogram of PCR with microsatellite IVS27AC28. A: PCR with DNA from peripheral blood of the father of the patient. B: PCR with DNA from peripheral blood of the patient. M indicates the maternal allele. C,D: PCR with DNA from microdissected melanoma cells showing LOH of the maternal allele (C) and a PCR product of reduced size (D, asterisk). Dotted lines indicate peaks and sizes of the molecular size marker GeneScan®-400 HD [ROX].
