Table 3.

Proportion of cells that were puromycin resistant after transduction reflected the dilution of the vector.

Experiment	Time after transduction (week)	Proportion of cells resistant to puromycin		Ratio of neat: ten fold dilution
		neat	1/10 dilution
1	1	0.189	0.0131	14.4
	5	0.0111	7.73 × 10-4	14.9
2	2	l.64 × 10-3	2.64 × 10-4	6.21

Two independent experiments were conducted. Different vector preparations were used to transduce Jurkat T cells. In each experiment, transduction was conducted using both the neat vector stock and a 1/10 dilution of the preparation. The proportion of cells that were puromycin resistant was determined, as indicated. The absolute proportions of cells resistant to puromycin were different, reflecting the fact that different vector stocks were used. However, within each experiment, the proportion of cells resistant to puromycin was 6.21 to 14.9 fold higher when the neat stock was used, broadly reflecting the 10 fold dilution of the vector preparation used in transduction.