Table 1.

Protease Inhibitor resistance of HIV-2 Proteases tested in yeast

HIV-2ROD sequence	Phenotype (virus production)		Phenotype (transformed yeast) (% cell growth)
	LPV	SQV	LPV	SQV
wild type	S	S	91.5+/-1.2	75.0+/-7.0
K45R	R [9]	R [9]	50.3+/-3.0	39.0+/-4.3
V20A, I54M	R [9]	R [9]	17.1+/-0.7	25.7+/-0.8
M76V	n.d.	R [10]	0.0+/- 2.4	4.0+/-0.2

The susceptibility of mutated HIV-2_ROD Proteases, harbouring amino acid substitutions known to confer a resistant phenotype, were assessed in yeast in the presence of LPV at 150 μM and SQV at 1mM.

The resistant (R) or sensitive (S) phenotype is referred to previously published data.

Results in the yeast system are presented as the percentage of cell growth; [(OD₆₀₀ cells grew in Galactose with PI – OD₆₀₀ cells grew in Galactose)/OD₆₀₀ cells grew in Glucose] × 100.