Table 1.
Antibodies used
Antibody | Raised in | Specificity | Dilution | Supplier |
---|---|---|---|---|
anti-TrkA | Rabbit | TrkA (residues 763–777) | 1:100 | Santa Cruz Biotechnology |
anti-TrkB | Rabbit | TrkB (residues 794–808) | 1:100 | Santa Cruz Biotechnology |
anti-TrkC | Rabbit | TrkC (residues 798–812) | 1:100 | Santa Cruz Biotechnology |
anti-NGF | Rabbit | recombinant NGF | 1:1000 | Chemicon, Inc |
anti-BDNF | Rabbit | recombinant BDNF | 1:1000 | Chemicon, Inc |
anti-NT-3 | Rabbit | recombinant NT-3 | 1:500 | Chemicon, Inc |
anti-S100 protein | Rabbit | α and β subunits of S100 protein | 1:1000 | Dako |
anti-NFP (clone 1592) | Mouse | Medium and high NFP subunits | 1:100 | Boehringer-Manhein |
BDNF: brain-derived neurotrophic factor; NFP: neurofilament proteins; NGF: nerve growth factor; NT-3: neurotrophin 3.