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Western blots showing immunoreactivity in homogenates of ED-12 lenses in regions 1, 2, 3 and 4. (a)Cytochrome c is detectable in regions 1 and 2, but not in 3 or 4. (b) Cytochrome oxidase is abundant in regions 1 and 2, still detectable in region 3, but undetectable in region 4. (c) Apaf-1 is detectable in regions 1 and 2, but not in 3 or 4. ‘J’ is a positive control lane using Jurkat cell homogenate.
