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Photomicrographs of envelopes of quail eggs. (a) An egg obtained at the magnum–isthmus junction. The vitelline membrane (VM) and peri-albumen layer (PL) are stained with Alcian blue. (b) Shell membrane (SM) from an ovulated egg. The PL at the interface between the egg white (EW) and SM is stained with Alcian blue. (c) Decalcified eggshell. The shell matrix (M) is stained with Alcian blue but the mammilla (m) is not. C, cuticle; Y, yolk. Stained with Alcian blue and eosin.
