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The anlage of the future digital bone at E13. Mesenchymal cell condensation (arrow in a) with no matrix metachromasia is seen by toluidine blue staining (TB). Versican mRNA (arrow in b) as well as immunostaining (arrow in c) are expressed in this mesenchymal cell condensation. There is strong HA staining in the mesenchyme around the condensation (arrowheads in d) but not in the mesenchymal condensation (arrow in d). Width of view = 100 µm.
