Figure 6.

Effect on CB1 agonists on endothelial [Ca²⁺]_i, P450 activity and cyclic AMP levels. (A) Basal [Ca²⁺]_i and the bradykinin (10 nM)-induced Ca²⁺ response in fura 2-labelled human endothelial cells. Experiments were performed in the absence (0.06% ethanol) and presence of HU 210 (30 μM). Results are presented as the mean±s.e.mean of eight separate experiments. (B) Ethoxyresorufin dealkylase activity was assessed in endothelial cells pre-incubated with either solvent, anandamide (30 μM), HU 210 (30 μM), Δ⁹-THC (30 μM) or miconazole (3 μM). Results are presented as the mean±s.e.mean of five separate experiments. (C) Intracellular cyclic AMP levels in endothelial cells under basal and isoprenaline (0.3 μM)-stimulated conditions. Experiments were performed in the absence and presence of HU 210 (30 μM) or Δ⁹THC (30 μM). Results are presented as the mean±s.e.mean of six separate experiments ^*P<0.05, ^**P<0.01, ^***P<0.001 vs the appropriate solvent.