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Semiquantitative expression of Cysteine Rich Intestinal Protein (CRIP): filters were dotted with spots of two genes, CRIP and Elongation Factor 1-α. The filters were probed with radiolabelled cDNA produced from five ganglia at chosen time points. Specific activity is measured using an NCBI image programme that determines how dark each spot was. This gives an assessment of the degree of hybridization, and hence a measure of expression level.
