Table 2. The four ganglion libraries constructed using whole nerve cords (conventional, non-PCR-based library) or whole regenerating ganglia at 1, 3 or 5 days after cutting major nerve roots (PCR-based libraries). The table shows percentage recombinants, size of the libraries and average size of the inserts.
library name | percentage recombinants | size of primary library (plaque forming units) | average insert size (bp) | |
---|---|---|---|---|
REHmGC | complete CNS | 91 | 3.75 × 104 | 700 |
REHmG9r24s | subtracted 24h post-axotomy | 93 | 3.05 × 105 | 700 |
REHmG9r72 | 72h post-axotomy | 98 | 1.36 × 105 | 600 |
REHmG9r120 | 120h post-axotomy | 97 | 1.22 × 105 | 500 |