Table 3. Selected clones from the 24-h ganglion subtracted library (REHmG9r24S in Fig. 6). One million plaque-forming units (pfu) of amplified total CNS library (REHmGC in Fig. 6) were screened using subtracted probes (24 h vs. sham-operated control) and 105 differentially hybridizing clones were isolated. Fifty-five of these were up-regulated at 24 h post-axotomy. The individual genes identified to date are listed. The nearest homology is shown together with the accession number of the human homologue.
| Gene ID | nearest homologue§ | gene name | size (bp) | human homologue¥ accession |
|---|---|---|---|---|
| Re24s1.1.1 | Danio rerio heat shock protein hsp90beta (AF068772) | HSP90 | 612 | AF028832 |
| various clones | H. medicinalis 16s ribosomal RNA (AF325971) | 16s rRNA | 319–607 | AF011168 |
| Re24s.42 | Cyanidium caldarium cytochrome oxidase subunit II (Z48930) | COX II | 600 | |
| Re24s26.1.1 | Zenopsis nebulosus cytochrome c oxidase subunit III (AP002942) | COX III | 441 | AF346965 |
| Re24s28.1.1 | Arabidopsis thaliana ubiquitin-conjugating enzyme (X68306) | UCE | 767 | I59365 |
| Re24s3.1.1 | Bos tarus b-1,4-N-acetylglucosaminyltransferase (AB000628) | GnT | 2031 | AB024911 |
| Re24s7.1.1 | Plectus acuminatus actin (AJ012665) | actin | 1353 | S38782 |
| Re24s.19 | H. medicinalis neuron-specific protein (AAB40925) | LeSPTR | 159 | |
| Re24s4.1.1 | novel 1 | 148 | ||
| Re24s17.1.1 | novel 2 | 416 | ||
| Re24s34.2.1 | novel 3 | 417 |
§
blastx of nr database at NCBI http://www.ncbi.nlm.nih.gov/BLAST/
¥
blastp of nr at NCBI using translated ORF