Table 1.
Identity, source and working dilution of antibodies used in this study
| Antibody | Clone | Supplier | Host and type | Antigen retrieval required | Dilution TBS |
|---|---|---|---|---|---|
| Mouse IgG1 (negative control) | IgG1 kappa | Dako Ltd | Mouse monoclonal | Yes | 1 : 50 |
| α smooth muscle (SMA) | 1A4 | Dako Ltd | Mouse monoclonal | No | 1 : 25 |
| Vimentin | V9 | Dako Ltd | Mouse monoclonal | Yes | 1 : 25 |
| Desmin | DE-R-11 | Dako Ltd | Mouse monoclonal | Yes | 1 : 50 |
| Cytokeratin 18 | DC10 | Dako Ltd | Mouse monoclonal | Yes | 1 : 50 |
| Cytokeratin (pan) | AE1 and AE3 | Dako Ltd | Mouse monoclonal | Yes | 1 : 50 |
| Transforming growth factor α (TGF-α) | 213–4.4 | Oncogene Research Products (Cambs., UK) | Mouse monoclonal | Yes | 1 : 100 |
| Follicle stimulating hormone-receptor (FSH) | Biogenesis (Poole, Dorset) | Sheep polyclonal | Yes | 1 : 100 | |
| Oestrogen receptor-β | 14C8-ab288 | Abcam Ltd | Mouse monoclonal | Yes | 1 : 50 |
| Oestrogen receptor-α NCL-ER-LH2 | CC4-5 | Novo Castra (Newcastle, UK) | Mouse monoclonal | Yes | 1 : 50 |
| Inhibin α subunit | R1 | Oxford Bio-Innovation Ltd | Mouse monoclonal | Yes | 1 : 100 |
| Inhibin Beta B subunit | C5 | Oxford Bio-Innovation Ltd | Mouse monoclonal | Yes | 1 : 100 |
| Androgen receptor NCL-Arp (AR) | Novo Castra | Rabbit polyclonal | Yes | 1 : 10 | |
| Ki67 (proliferation marker) | Novo Castra | Rabbit polyclonal | Yes | 1 : 250 | |
| Alpha-Tubulin (sea-urchin) | B-5-1-2 | Sigma | Mouse monoclonal | No | 1 : 100 |
| Tubulin (yeast) | YOL1/34 | Abcam (Cambs., UK) | Rat monoclonal | No | 1 : 400 |
| Transcription factor GATA-4 | C-20 | Santa Cruz Biotechnology | Goat polyclonal | Yes | 1 : 200 |