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Mean percentage of PCNA-positive Sertoli cells cultured on ECM gel-coated substrate: (A) E12.5 cells cultured for 2 div; (B) E12.5 cells cultured for 7 div; (C) E13.5 cells cultured for 3 div; (D) E13.5 cells cultured for 7 div; (E) E14.5 cells cultured for 7 div. Data were analysed using Kruskall–Wallis analysis and Dunn's post hoc analysis. *Significantly different from 0 ng mL−1 GDNF cultures, P < 0.05; **significantly different from 0 ng mL−1 GDNF cultures, P < 0.01.
