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mRNA expression of bax and bcl-2 in antler tissues. (A) β-actin and (B) bax (289 bp) and bcl-2 (201 bp) were detected by multiplex RT-PCR. RT-PCR products were resolved by agarose gel electrophoresis and stained with ethidium bromide. MW = 100-bp molecular weight; 1 = skin; 2 = perichondrium/mesenchyme; 3 = non-mineralized cartilage; 4 = mineralized cartilage. Bax expression was higher than that of bcl-2.
