Fig. 1.

NG2-expressing spinal cord progenitors express astrocyte and neural markers. Spinal cord progenitors isolated from adult mice are maintained in vitro and fractionated according to NG2 expression via flow cytometry. The growth mediium is then supplemented to induce differentiation. (A) NG2-expressing cells differentiate into GFAP (green)-expressing astrocytes when grown in media supplemented with 1% FBS. (B) Under neural differentiation conditions (0.5 µmtrans-retinoic acid, 10 µg mL⁻¹ BDNF), NG2 progenitors differentiate and express neural proteins (TuJ1, blue). Scale bar = 10 µm.