Figure 6.

Reverse Transcription Polymerase Chain Reaction (RT–PCR) of mRNA isolated from VSMC after 16 h incubation. All samples containing PCR products were incubated in triplicate. (A) Photograph of an agarose gel in which lanes 1–3 are Controls, lanes 4–6 are samples treated with media containing 50 μg ml-1 LPS, lanes 7–9 samples treated with cyclosporin A (CsA, 1 μM) alone, lanes 10–12 samples co-incubated with LPS and 10 μM CsA, lanes 13–15 LPS and 1 μM CsA and lanes 16–18 LPS and 0.1 μM CsA. Expected PCR product sizes are 636 bp for NOS2 and 212 bp for β-actin. (B) The relative density of bands of PCR products shown as ratios of NOS2 and β-actin products. Results are means±s.e.mean of three experiments.