Figure 3.

Time-course of the effects of nepalolide A on the accumulation of nitrite in C6 glioma cells. C6 glioma cells were incubated with or without 10 μM nepalolide A for 30 min. After 30 min incubation, cells were challenged with 5 μg ml⁻¹ LPS/100 units ml⁻¹ IFN-γ (a) or 5 ng ml⁻¹ TNF-α/5 ng ml⁻¹ IL-1β/100 units ml⁻¹ IFN-γ (b) in the presence or absence of nepalolide A for the times indicated. After these incubation periods, the culture media were collected for nitrite determination. Results are means±s.d. (where large enough to be shown) from four independent experiments. Significant differences between control and nepalolide A-treated cells are indicated by ^*P<0.05; and ^***P<0.001.