Figure 6.

Dose dependent inhibition of cytomix-induced NOx production by NF-κB inhibitors in Caco-2 cells. Caco-2 cells (passages 32 to 39) were used 10 days after confluence. They were treated in serum free medium for 24 h with cytomix alone or with increasing concentrations of pyrrolidine dithiocarbamate (PTDC) (a) and 3,4 dichloroisocoumarin (DCI) (b). NOx level was determined in the supernatant by nitrate reduction and the Griess reaction. Results are expressed as percentage of maximal induction (cytomix alone) and represent means (±s.e.mean) from at least three different experiments, each done in triplicate. ^*P<0.05, ^**P<0.01, ^***P<0.001 compared with value obtained with no inhibitor.