Figure 6.

Effect of indomethacin on the regulation of COX-2 mRNA in carrageenan-induced paw ædema. Indomethacin (10 mg kg⁻¹ p.o.) or vehicle (methocel) was given orally 1 h prior to intraplantar injection of carrageenan. Ipsilateral (injected) and contralateral (non-injected) paws were taken at indicated time, mRNA was isolated and both COX-2 and β-actin mRNA levels analysed by RT–PCR. Data are expressed as fold±s.e.mean of the intensity of the COX-2-specific band over that of β-actin obtained from the injected and non-injected paws of eight animals. ^*P<0.05 between ipsilateral and contralateral curves. #P<0.05 between data obtained at time zero and in naive animals.