Figure 2.

HPLC analysis of Ip₅I production. (A) a series of chromatographic profiles for the time-dependent enzymatic conversion of Ap₅A into the intermediary product Ip₅A and final product Ip₅I by adenylate deaminase (Aspergillus sp.) over a period of 90 min (chromatograms at: T=0, 15′, 30′, 45′, 60′ and 90′). (B) a series of chromatographic profiles for the time-dependent production of IMP and Ip₄ by phosphodiesterase breakdown of the reaction product Ip₅I (chromatograms at: T=0, 5′, 10′, 15′ and 30′). Ordinate scalars (A and B) as AUFS (absorbance units full scale), as defined by Millennium 2010 software (Waters).