Figure 6.
Effect of Me5 on open probability of sodium channels in the presence of ATX in a cell-attached patch of rat skeletal muscle fibres. (A, B) Sodium currents elicited during the time interval 10–145 ms of eight consecutive depolarizing pulses of 150 ms in duration applied to −20 mV from a holding potential of −70 mV, in the presence of 5 μM ATX, before and after application of 10 μM Me5. Stimulation frequency was 1 Hz. (C, D) Ensemble average currents constructed from 200 current traces elicited as in (A) and (B). (E) Diary of sodium channel open probability (NP0) during 100 consecutive depolarizing pulses used for the ensemble average currents shown in (C) and (D). The means±s.e.mean of NP0 calculated before and after application of Me5 are reported.