Figure 7.

Effect of Me5 on single-channel current amplitude and conductance in the presence of ATX in cell-attached patches of rat skeletal muscle fibres. (A,B) All-points amplitude histograms constructed at −20 mV from current traces elicited as in Figure 6A (same patch), before (A) and after (B) application of 10 μM Me5. Sampled data (open circles) were fitted to a three-Gaussian function in (A) and a two-Gaussian function in (B). (C) Single-channel current–voltage relationships constructed from single-channel current amplitudes determined either by all-points amplitude histograms as in (A) and (B) or by direct measure on square-shaped single-channel events. Each data is the mean±s.e.mean from four patches. Data collected before and after application of 10 μM Me5 were linearly correlated (P<0.001 for both), indicating single-channel conductances of 17.4 and 17.3 pS, respectively.