Figure 3.
Glibenclamide induced block of outward currents in human atrial myocytes. (a) Total outward current in response to a depolarization to +40 mV under control conditions and under the influence of glibenclamide (30, 100 μM). Glibenclamide affected both, the peak current and the late current at the end of the clamp pulse. The time-course of inactivation of the total outward current was accelerated in the presence of glibenclamide. (b) Current voltage relationship of the peak total outward current under control conditions and under 100 μM glibenclamide (n=5). (c) Mean concentration-effect data of the effect of glibenclamide on charge area beneath total outward current. The solid line represents the results of fitting a Hill function to experimental data giving an IC50 76.4±6.3 μM and a slope of −1.21±0.13. (*P<0.05).