Figure 3.

Effects of BDNF or SR 57746A on survival of purified mouse motoneurons. Purified mouse motoneurons were incubated at 37°C with BDNF (100 ng ml⁻¹) or SR 57746A (1 μM) immediately after seeding. For control cells, DMSO (0.02%) was added to the culture medium instead of the drugs. Motoneuron counting was performed at 24, 48, 96 and 120 h as described in Methods. Data shown are the ratio (percentage of surviving motoneurons in treated cultures compared to control cultures) ±s.e.mean for four independent experiments, each conducted with duplicate determinations. ^*P<0.05 compared to control (ANOVA one way test, followed by Fisher test for multiple comparisons).