Figure 4.

Effects of SR 57746A and/or genistein on survival of purified mouse motoneurons. Purified mouse motoneurons were incubated at 37°C with SR 57746A (1 μM) and/or genistein (10 μM) immediately after seeding. For control cells, DMSO (0.02%) was added to the culture medium instead of the drugs. Motoneuron counting was performed at 24, 48, 96 and 120 h as described in Methods. Data shown are the ratio (percentage of surviving motoneurons in treated cultures compared to control cultures) ±s.e.mean for three or more independent experiments, each conducted with duplicate determinations. ^*P<0.05, ^**P<0.01 compared with control (ANOVA one way test, followed by Fisher test for multiple comparisons).