Figure 4.

Effect of I-R on mRNA expression of COX-1 and COX-2. Total RNA was extracted from gastric mucosa of sham operated rats (Sham op, lane 1), rats subjected to 30 min ischaemia and 60 min reperfusion (1.5 h, lane 2) or 30 min ischaemia and 5.5 h reperfusion (6 h, lane 3). The autoradiograph shows a representative Northern blot analysis hybridized with cDNAs specific for COX-1, COX-2 and GAPDH (as internal standard) (A). Relative signal intensities of COX-1 and COX-2 to that of GAPDH were quantified and the ratio of sham operated rats was set to 100% (B). The values are expressed as mean±s.e.mean (n=3); ^*P<0.05 vs sham operated rats.