Release of [3H]-serotonin from isolated rat brain stem before and after electrical stimulation, in the absence and presence of PEA, tryptamine and (−)BPAP, respectively. (N=8). Each column represents the amount of [3H-]-serotonin in picomoles released in a 3 min collection period. Vertical lines show s.e.m. Paired Student's t-test. *P<0.05, **P<0.02, ***P<0.01, ****P<0.001.