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Expression of productive Cε mRNA in cervical lymph nodes of BALB/c and C57BL/6 IL-4 gene-depleted and wild type mice. Mice received a topical application of 0.15% of DNFB in acetone and olive oil (vehicle) on the ears once a week for 5 weeks. Cervical lymph nodes were obtained 4 h after the fifth application. The expression of productive Cε mRNA was examined using RT–PCR. M: size marker, V: vehicle, D: DNFB.
