Figure 4.
Steady-state inactivation and voltage-dependent conductance of ICa,L. (A) Left panel: a double-pulse protocol, 1-s conditioning pulses to various voltages (−80∼0 mV in increments of 10 mV) from HP of −80 mV followed by a fixed test pulse to 0 mV for 300 ms, which was applied to examine the steady-state inactivation of ICa,L. Right panel: typical traces showing ICa,L at different steady inactivation states. (B) Effects of Pro on ICa,L steady-state inactivation and conductance-voltage relationship. Smooth curves are Boltzmann fittings for control (solid line) and Pro-treated (dashed line) groups. (See methods for details on calculation of I/Imax, g/gmax and Boltzmann fitting). n=7 for each data.