Figure 3.

Pharmacological profile of [³H]-glyburide binding in transfected HEK-293 cells (A) Displacement of specific [³H]-glyburide binding by sulphonylurea analogues. Assays were performed using 0.3 nM of [³H]-glyburide with varying concentrations of various sulphonylurea analogues as described in Methods. (B) Correlation of the K_i values in transfected HEK 293 cells with those observed in RINm5F (r²=0.97; slope=1.05±0.07) and (C) with rat brain (r²=0.93; slope=1.03±0.11) membranes. The K_i values were calculated from the IC₅₀ values using the Cheng-Prusoff equation as described in Methods.