Figure 6.

Detection of pp60^c-src by Western blot analysis of rabbit ear artery cell lysates. Cell lysates were prepared from freshly isolated rabbit ear artery cells as described in the Methods. 15, 30 and 50 μg of protein were run on a 10% polyacrylamide gel and immunoblotted with an anti-c-Src antibody (Mab327, 2.5 μg ml⁻¹). Proteins were detected using an HRP-linked secondary antibody followed by enhanced chemiluminescence. Figure shown is representative of three separate experiments. The position of the molecular weight markers are shown on the right. The band marked with an arrow corresponds to a protein with an approximate molecular weight of 60 kDa identified by the anti-c-Src antibody.